Sefa yekuverenga ye microplate

Magirazi eBodian ane ruzivo rwemakore akawanda mukutsvaga nekugadzikisa firita. Firita rekuverenga re microplate rakapihwa rine zvakanakira zvekuisa wavelength chaiyo, chiyero chepamusoro chesignal-to-noise, kugadzikana kwakanaka kwezvakatipoteredza uye kusafamba-famba. Takagadzira mafirita epasi rose nemafirita anoenderana nesimba zvichienderana nezvinodiwa nemushandisi, ayo akakodzera mhando dzakasiyana dzevaverengi ve microplate.

Kushandiswa:

Microplate reader chishandiso chehunyanzvi chekuverenga nekuongorora mhedzisiro yekuedza yeenzyme-linked immunoassay (EIA). Inoshandiswa zvakanyanya mukuyedza kwekiriniki, kutsvagisa kwezvipenyu, sainzi yekurima, sainzi yechikafu nesainzi yezvakatipoteredza. Sechishandiso chikuru che monochromatic munzira yekuona ye microplate reader, firita yakakosha zvikuru kune kurongeka, kudzokorora uye kugadzikana yemhedzisiro yekuyerwa kwekunyudzwa kwemvura.

Magadzirirwo eFirita:

Sefa ye microplate reader inowanzo tsanangurwa zvichienderana nehuwandu hwe wavelength hwavanotumira, passband yavo. Kunze kwe passband wavelength, zvinhu zvitatu zvakakosha ndeizvi kureba kwepakati (CWL), iyo upamhi hwakazara pahafu yepamusoro (FWHM)uye kutapurirana kwepamusoro-soro (T).

Hwande hwepakati (CWL) hwe microplate reader filter ndiyo wavelength iri pakati pepassband. Kutumirwa kwepamusoro (T) ndiyo wavelength yechiedza pakutumira kwepamusoro. Pakupedzisira, upamhi hwakazara pahafu yepamusoro (FWHM) ndiyo bandwidth iri pa50% yekutumira kwepamusoro.

Dzimwe nguva mawavelength akachekwa uye akachekwa anoshandiswa kutsanangura passband yefirita panzvimbo peFWHM neCWL. Kuchinja kubva papassband kuenda kumawavelength akavharika echiedza kunotsanangurwa nekukwira kwemucheto. Kana firita ine mupendero wakakora, transmission inodonha nekukurumidza pamawavelength akachekwa uye akachekwa.